RPK-16 was created by Shaw alongside AK-12, AN-94 and AK-15 as a member of Team DEFY with Angelia as their leader.
Her obsession with humanity and her desire to learn of their emotions and lives caused her to act erratically cruel, ambitious and eventually treacherous when she betrayed DEFY and joined forces with Paradeus without any sense of remorse and regret. She is also arrogant and confident, having mocked her opponents while using cheap tricks to defeat them.
RPK-16's desire of wanting to feel pain, love, desires just like humans also indicated that simply living was not her end goal, having intimated that she is a prisoner within her own Neural Cloud due to how Shaw designed her and that true death fascinated her.
It was later revealed that the cause of her personality was that she was working as a spy for Griffin Lyons, as she joined Paradeus to use them as pawns for her superior's plan.
At a Paradeus based referred to as Avernus, RPK was seen communicating with a Nyto named Koleda, telling her to take good care of the captured Angelia. With Koleda's true identity as AK-12 under deep cover, it was hinted—though not confirmed—that RPK may still be acting in Angelia's best interests by acting as a mole.
RPK later awoke in a new, seemingly organic Nyto-esque body only to be confronted by M16A1. The two engaged in a lengthy fight which ended with RPK heavily wounded from a point-blank explosive and unable to stop M16A1 from leaving to rescue RO635, leaving RPK-16 to muse on the nature of her and M16A1's bond before cryptically stating that her modifications will soon be complete.